Pages: 17-23

Antimicrobial activities of ethyl acetate extracts of fungi isolated from waste dump sites in Ado-Ekiti, Nigeria

Author: J O Oluyege, P I Orjiakor and A R Omolara

Category: Biology

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Abstract:

The search for natural antimicrobial agents continues unabated to antimicrobial agents such as antibiotics are one of the most important exploited secondary metabolites of microorganism and therapeutic purposes. Considering the clamor for search of more potent and broad spectrum antimicrobial agents, this study was carried out to investigate the antimicrobial production potentials of fungal isolates from the waste dump sites within Ado- Ekiti, Ekiti State, Nigeria. The mean fungal count in all the dumpsite ranged from 5.7 x 104 CFU/g to 2.1 x 106 CFU/g. Among the predominant fungal isolates, Aspergillus, Cladiosporium, Penicillum and Trichoderma species were selected for antibacterial activities against based on the screening test. The test bacteria (Staphylococcus aureus, Klebsiella pneumoniae, Bacillus sp., Escherichia coli, Pseudomonas aeruginosa, Serratia sp. and Streptococcus sp.) were also isolates from the dumpsites. The ethyl acetate extract (50 mg / mL) of each fungus inhibited the growth of at least three of the test pathogens. The antimicrobial effects of Aspergillus, Trichoderma, Cladosporium and Penillium extracts ranged from 10-16.0 mm, 9.0-15.0 mm, 5.0-14.0 mm and 6.0-16.0, respectively. The most susceptible bacteria were E. coli, followed by S. aureus, K pneumoniae and Bacillus sp. Serratia sp. the least, being inhibited by only Trichoderma sp. extracts. Ciprofloxacin drug (30 μg) gave antibacterial inhibition of 10.0 - 48.0 mm, with K. pneumonia and P. aeruginosa being the least and most susceptible bacteria. Findings from this study suggest that Aspergillus, Penillium, Trichoderma and Cladosporium possess marked in vitro antimicrobial biomolecules that could be considered for antibiotic production. Purification of the bioactive molecules is essentials.

Keywords: Antimicrobial agents, fungi, bacteria, Ciprofloxacin


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