Pages: 24-30

Cell-free formulation obtained from Solid state fermentation for effective alleviation of Hydrophobic soil sample

Author: R. Nandhini, S. Sridhar

Category: Biology

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Abstract:

Soil is the largest superficial global carbon reservoir, is made up of organic compounds and includes plant, animal and microbial material, both living and dead. Soil water repellency (SWR) is some phenomena in which soil does not spontaneously wet when a drop of water is applied to the surface, occurs due to the formation of hydrophobic organic coatings on soil particle surfaces indicating that the soil is hydrophobic. Solid state fermentation (SSF) is an eco-friendly, cost effective and widely available agricultural residues were used as substrate for enzyme production. Among various solid substrates, wheat bran has been found to be effective substrate for SSF for production of cell free extra-cellular fractions. The main objective of our current work is to test the efficacy of crude enzymatic formulations to alleviate hydrophobicity in the soil. The present study was undertaken to produce the crude enzyme by A. fumigatus and E. coli on wheat bran as solid support in Solid state fermentation to analyze the water repellent soils by using Water drop penetration time (WDPT analysis) and the Molarity of Ethanol Droplet Method (MED value). Among these methods WDPT showed positive results towards water repellency. The current studies of WDPT and MED can be recommended for future studies in the arena of surface water repellent soils.

Keywords: Soil water repellency, Wheat bran, Solid State Fermentation, Hydrophobic soils, Crude enzyme, MED and WDPT


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