Pages: 92-101

Apoptotic induction by Cassia fistula leaf extracts against human hepatocarcinoma cell lines

Author: H A Ssadh, S Srinivasan, I H Madar, I A Tayubi

Category: Biology

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Abstract:

Cassia fistula has therapeutic importance in the field of health care. Several studies reported the use of this plant for the treatment of abdomen, glands, liver and throat cancer indicating its high efficacy and medicinal value. This study was performed to identify the anti-cancer property of traditional medicinal herb, Cassia fistula on Hep G2, human hepatic carcinoma cells. The cytotoxicity potential of the plant extract on HepG2 cells was analysed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay followed by determination of its genotoxicity potential by DNA fragmentation assay. Increased level of cysteine-aspartic proteases (CASPASEs) quantified in treated cells validated the DNA fragmentation assay and confirmed the induction of apoptosis. The leaf extracts were prepared using hexane, chloroform and methanol as solvents and subjected to phytochemical investigation. Reverse transcriptase polymerase chain reaction (RT - PCR) suggested the involvement of mitochondrial proteins in the pathway. The database search and text mining approaches performed using liver cancer cells revealed the apoptotic role of B-cell lymphoma (Bcl-2) gene. Results showed that chloroform extract of the plant leaves was most toxic to cancer cells. Further, Bcl-2 gene expression was observed to be low in cells treated with the chloroform extracts of C. fistula leaves, suggesting an involvement of mitochondrial proteins in the HepG2 cell apoptosis. Hence, herbs like Cassia fistula being a part of traditional medicine in India can also aid as an effective anti-cancer therapy to combat this deadly disease.

Keywords: Cassia fistula, Liver cancer, Cytotoxicity, HepG2 cells, Bcl-2 gene, CASPASEs


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