Pages: 107-112

Study of the cytotoxic effect of Calotropis procera on Breast Cancer Cell Line, T47D in Vitro: A Traditional Remedy

Author: Hassan Swed Alzahrani, Safar A. Almalki, Mohamed R. Rizgallah

Category: Biology

Abstract:

Calotropis procera, a plant from Apocynaceae family has been shown to have anticancer properties in several studies. This plant is found in tropical Africa and several parts of Asia. Using methanol extraction method, we characterized the extracts of leaves of Calotropis procera using FTIR and UV-VIS spectrophotometry. The extracts obtained from C. procera leaves were investigated for its anticancer ability against a breast cancer cell line T47D (ATCC® HTB133™). We carried out a colorimetric assay known as MTT assay using dye compound 3-(4,5-Dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide for assessing cell metabolic activity. This study suggests that methanolic fraction of C. procera extracts proved effective against the proliferation of breast cancer cell line T47D. With our results, we can impart positive emphasis on the importance of metabolic fraction of leaves of C. procera in inhibiting the cell proliferation of cancer cells.

Keywords: Calotropis procera, breast cancer, T47D, UV-VIS, Giemsa Stain


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